Protective effects of Digera muricata (L.) Mart against Liver toxicity Protective effects of methanolic extract of Digera muricata (L.) Mart. against toxicity by acrylamide in rat liver

This experiment was conducted to determine the protective effects of methanolic extract of Digera muricata ( L.) Mart. on the acrylamide induced injuries in liver tissues of rats at the histology and biochemical levels. Forty eight healthy female albino rats, weighting 190-200 g, were provided by the Animal House of National Institute of Health (NIH) Islamabad and were maintained at the Primate Facility maintained at Quaid-i-Azam University, Islamabad. These rats were divided into eight groups with six rats in each group. Among them group I remained as control, while group II was treated with DMSO 5.0 ml/kg B.w.(Body weight) orally once a day for five weeks. Group III was given 200 mg/kg B.w. of methanolic extract of Digera muricata dissolved in DMSO once a day for five weeks. Rest of the rats were divided into five groups and were treated with aqueous solution of acrylamide 6 mg/kg B.w. intraparetoneally once a day for two weeks. Group IV was sacrificed after the acrylamide treatment to collect the serum and liver. Group V remained untreated as such for the rest of the experiment. Group VI, VII and VIII were given 100, 150 and 200 mg/kg B.w. methanolic extract of Digera muricata