In vitro regeneration of Acacia mangium Willd. pathways for mass multiplication

Present investigation developed three pathways for in vitro regeneration and mass multiplication of Acacia mangium. The nodal segments from 8-10 years old phenotypically superior plus tree were used as explants for axillary bud culture. MS medium supplemented with BAP and Kinetin in combination was the best for culture establishment and shoot proliferation from the axillary buds. Microshoots were rooted in MS medium (half strength) supplemented with IBA and acclimatization of the rooted shoots was obtained in plastic pots containing soilrite. Sixty per cent of cotyledon explants developed direct adventitious buds. Callus was induced from the cotyledon and hypocotyl explants on MS medium fortified with NAA and BAP. Genetic fidelity of micropropagated plants was examined by RAPD analysis, at every subculture. No genetic variation was detected among plantlets, hence proving genetically stable regeneration method. This protocol can be very useful for for genetic engineering and mass production of quality planting material of Mangium.